Apply to the whole head of hair every night, starting first with the scalp region where hair loss is seen and the surrounding area. To increase the efficacy of the spray, apply twice a day, once in the morning and once in the evening. Make sure the spray is fully absorbed by the scalp by gently massaging in a circular motion for at least one minute. Do not rinse afterward. BIOXSINE Forte Spray can be used day or night and should be kept on the hair for a minimum of eight hours. To get even better results, use regularly with BIOXSINE Forte Shampoo.